gregmartin
Seed
backyard breeding for zone 5 hardy perennial edible landscaping in Maine
Posts: 17
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Post by gregmartin on Jan 25, 2015 8:20:52 GMT -5
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Post by darrenabbey on Jan 25, 2015 10:25:17 GMT -5
Interesting... and it does describe the ploidy of specific varieties. Thanks!
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Post by nicolas on Jan 27, 2015 2:27:11 GMT -5
Something that could be helpfull, extracting strawberry DNA in a c.o.c.k.tail
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Post by darrenabbey on Jan 27, 2015 19:02:46 GMT -5
That would be the process if I wanted to do some genome sequencing... which might be an ideal step for confirming the hybrid nature of the target plant.
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Post by papabearjay on Feb 23, 2015 20:08:32 GMT -5
That would be the process if I wanted to do some genome sequencing... which might be an ideal step for confirming the hybrid nature of the target plant. So, I am taking requests for "common" vegetables, herbs, and flowers which people would like to see experimented with in regards to chromosome-doubling. I'm trying to think of an effective population size as well, but I'm also limited by whatever comes in a seed packet. I have a number of things available and will compile a list but would be open to suggestions. I'd like to start with a known quantity before I venture into more difficult subjects though.
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Post by darrenabbey on Feb 24, 2015 3:10:47 GMT -5
The proposed project would still be a few years out before chromosome-doubling would be needed, but I might have some other projects... I'll have to ponder on the subject.
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Post by papabearjay on Feb 25, 2015 6:59:22 GMT -5
The proposed project would still be a few years out before chromosome-doubling would be needed, but I might have some other projects... I'll have to ponder on the subject. My concern here is how to approach the woody perennials, as they generally require stratification. Should the stratification occur before or after treatment? I don't want to wait 2 months to find out all the seed died or didn't convert, but I also don't want to wait 2 months to find out they all germinate but then die in response to the treatment....
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Post by darrenabbey on Feb 27, 2015 0:38:52 GMT -5
Stratification should be first. You want the seed to be germinating and metabolically active before attempting chromosome doubling. If the seed hasn't woken up yet, there won't be dividing cells for the chemical to interfere with.
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Post by papabearjay on Mar 1, 2015 8:52:28 GMT -5
This is not true in all cases, and sometimes stratification is necessary as a result of physical barriers (e.g. seed coat). These seeds may readily germinate without a physical barrier.
After the seed(s) has imbibed water it is physiologically active but there may be inhibitors present in the seed coat which must be degraded and is done so at specific temperatures.
An easy experiment to test given enough seed I suppose, though I imagine it might be genus/species specific.
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Post by Graham on Mar 26, 2015 17:38:58 GMT -5
As I think I've mentioned, I've wanted to see Skirret doubled for some time. Silverweed may be an interesting one to along with Chinese Artichokes. Just to see if larger roots/rhizomes result.
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Post by Steve on Jul 17, 2017 9:23:05 GMT -5
I have used Surflan (oryzalin) in home chromosome doubling experiments. It does work, and one can find papers where it worked better than oclchicine in some polyploidization projects.
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